Cellular and Biomolecular Analysis

Advances in genomic and proteomic methods now allow classification of disease based on molecular profiling. The detection of a molecular analytes and use of this type of information for disease diagnosis requires methods with superior sensitivity and specificity, along with high-throughput. We are developing new analytical methods with these properties that will permit the direct readout of nucleic acid sequences and protein biomarkers. Chip-based sensors made from nanomaterials play an important role in this effort, as detection sensitivity is greatly enhanced when measurements are performed at the nanoscale. We have also recently developed devices that capture rare cells with high efficiency.  Our aim is to generate detection systems applicable to the diagnosis of cancer, infectious disease and other disease states.

Featured publications:

"DNA Clutch Probes for Circulating Tumor DNA Analysis."J. Das, I. Ivanov, E.H. Sargent, S.O. Kelley, Journal of the American Chemical Society, 2016, 138, 11009-11016. link 

"An Electrochemical Clamp Assay for Direct, Rapid Analysis of Circulating Nucleic Acids in Serum.” J. Das, I. Ivanov, E.H. Sargent, S.O. Kelley, Nature Chemistry, 2015, 7, 569-575. link   

"Advancing the Speed, Sensitivity and Accuracy of Biomolecular Detection with Multi-Length Scale Engineering.” S.O. Kelley, C.A. Mirkin, D.R. Walt, R.F. Ismagilov, M. Toner, E.H. Sargent, Nature Nanotechnology, 2014, 9, 969-980. link

"An Ultrasensitve, Universal Detector Based on Neutralizer Displacement." J. Das, K.B. Cederquist, P. Lee, E.H. Sargent, S.O. Kelley, Nature Chemistry, 2012, 4, 642. link

"Direct Genetic Analysis of Ten Cancer Cells: Tuning Molecular Probe Design for Efficient mRNA Capture" E. Vasilyeva, Z. Fang, M. Minden, E.H. Sargent, S.O. Kelley, Angewandte Chemie, 2011, 50, 4137. link

"Hierarchical Nanotextured Microelectrodes Overcome the Molecular Transport Barrier to Achieve Rapid, Direct Bacterial Detection"  L. Soleymani, Z. Fang, B. Lam, X. Bin, E. Vasilyeva, A. Ross, E.H. Sargent, S.O. Kelley, ACS Nano, 2011, 5, 3360. link

"Programming the Detection Limits of Biosensors through Controlled Nanostructuring. "  L. Soleymani, Z. Fang, E.H. Sargent, S.O. Kelley, Nature Nanotechnology, 2009, 4, 844. link